Determination of SOD, POD, PPO and CAT Enzyme Activities in Rumex obtusifolius L.
Esma Hande Alici
Department of Chemistry, Sakarya University, Sakarya, Turkey.
Gulnur Arabaci *
Department of Chemistry, Sakarya University, Sakarya, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Aims: The purpose of this study was to measure antioxidant enzyme (polyphenol oxidase, peroxidase, catalase and superoxide dismutase) activities of crude extract of Rumex obtusifolius L. in order to gain insight about this plant’s antioxidant potential.
Study Design: The study was composed of the collection of plant material, extractions of the antioxidant enzymes, activity measurements of the enzymes and finally evaluation of the experimental results.
Place and Duration of Study: Department of Chemistry (biochemistry laboratories), Faculty of Science and Arts of Sakarya University, between June 2015 and July 2015.
Methodology: Enzymatic antioxidant activity of this plant was investigated by carrying out catalase, superoxide dismutase, peroxidase and polyphenol oxidase enzyme activity assays. Enzyme activities of the crude extract were measured by using spectrophotometric method. Optimum pH and temperature values of each enzyme were also determined for measurement of enzyme activities in ideal conditions.
Results: Finally, our results showed that Rumex obtusifolius L. crude extract had good activity for all the enzymatic procedures tested. The activity levels of enzymatic antioxidants polyphenol oxidase, peroxidase, catalase and superoxide dismutase of the plant were found to be 12.8; 195.2; 38.7; 11.6 EU/mL, respectively. Optimum pH and temperature values of all the enzymes (except PPO: optimum temperature 30°C) tested were also found to be 7.0 and 25°C, respectively.
Conclusion: Our results demonstrate that this edible plant, Rumex obtusifolius L., might be a potential source of natural antioxidants with good antioxidant enzyme capacity.
Keywords: Antioxidant enzyme, catalase, peroxidase, polyphenol oxidase, Rumex obtusifolius L., superoxide dismutase