Evaluation of Inhibition of Fungal Spore Germination by Rhizospheric Bacterial Extracts
Asma Benslim
Laboratory of Microbial Ecology, Faculty of Nature and Life Sciences, University Abderrahmane Mira, Béjaia, Algeria.
Samia Mezaache-Aichour *
Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Sétif 1, Sétif, Algeria.
Nora Haichour
Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Sétif 1, Sétif, Algeria.
Sami Chebel
Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Sétif 1, Sétif, Algeria.
Mohamed Mihoub Zerroug
Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Sétif 1, Sétif, Algeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: This work aims to evaluate the anti-germinative activity of bacterial extracts. These bacteria were already characterized for their antagonistic capacity in vitro against telluric phyto-pathogens fungi: Fusarium oxysporum f.sp. albedinis (Foa), Fusarium solani var. coeruleum (Fsc) and Phytophthora infestans (Pi), causing Bayoud, dry rot and mildew diseases respectively.
Methodology: The OD of bacterial cultures is measured in order to determine the microbial charge producing the anti-germination substances, then centrifuged, filtered. A volume of spore suspension of determined concentration is added to a determined volume of the bacterial filtrates. After incubation, for 24 hours at room temperature the inhibition of spore germination is observed under a microscope using a Malassez cell.
Results: The results showed that these bacterial filtrates are able of inhibiting fungal spore germination, among these filtrates XI35 ' one, gave a total inhibition (100%) on both of the tested fungi Foa and Fsc, while on Pi it only gave 16.66% of inhibition.
Conclusion: The bacterial filtrates were more active against mitosporic fungi, than oomycetes.
Keywords: Antagonistic bacteria, inhibition of germination, secondary metabolites, telluric phyto-pathogenic fungi