Ethnobotanical, Phytochemical and Biological Studies of a Medicinal Plant Recipe Used in the Treatment of Cervical Cancer
Françoise Eliane BONKIAN *
Department of Biochemistry and Microbiology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso and Laboratory for Research and Education in Animal Health and Biotechnology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
KOUDBI Jacob ZONGO
Laboratory of Molecular Biology and Genetics Training and Research Unit in Life and Earth Sciences. Joseph KI-ZERBO University, 09 BP 848 Ouagadougou 09, Burkina Faso.
Armandine LEMA
Department of Biochemistry and Microbiology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso and Laboratory for Research and Education in Animal Health and Biotechnology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
Koudougou Denis KARAOGO
Department of Biochemistry and Microbiology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso and Laboratory for Research and Education in Animal Health and Biotechnology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
Oivier B. BAMA
Department of Biochemistry and Microbiology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso and Laboratory for Research and Education in Animal Health and Biotechnology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
Hermann Yempabou OUOBA
University Joseph Ki-Zerbo, Life and Earth Sciences Training and Research Unit (UFR-SVT), Laboratory of Plant Biology and Ecology (LABEV), University of Nazi BONI, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Filkpièrè Léonard DA
Université Norbert ZONGO, Animal Physiology BP: 376 Koudougou, Burkina Faso.
Mindiédiba Jean BANGOU
Department of Biochemistry and Microbiology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso and Laboratory for Research and Education in Animal Health and Biotechnology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study was initiated to identify the medicinal plant recipes used in the management of the cervix (i) to carry out phytochemical screening (ii) and to evaluate some biological potentialities of the most cited recipe (iii).
Place and Duration of Study: These studies were carried out in Burkina Faso in the ecotoxicology of natural substances laboratory of INERA/Bobo and in the Life and Earth Sciences laboratory of Norbert ZONGO University during September 2023 and July 2024.
Methodology: Recipes were identified using survey sheets. The most frequently cited recipe was used for tube characterization tests and quantification tests using the spectrophotometer. Anti-inflammatory activity was determined by the paw immersion method using a plethysmometer after formalin injection (1%) and acute toxicity was assessed according to OECD Guideline 423.
Results: A total of 112 traditional practitioners were surveyed; 30 medicinal species composed of 21 recipes were identified. Tube characterization tests allowed the identification of tannins, alkaloids, saponons in the extracts. Quantification tests allowed obtaining respectively 1.294 ± 0.011 mg EQ /100mg and 24.723 ± 1.071 mg EAG /100mg in flavonoids and total phenolic compounds. The inhibition percentages of 35.81% and 43.80% were obtained by performing the anti-edematous test at the first hour at doses of 25 and 50 mg/kg also, the extracts showed no signs of toxicity at the dose of 300 mg/kg b.w.
Conclusion: Given these results, the recipe could be used on a line of cervical cancer cells to evaluate their effects on these cells.
Keywords: Cervical cancer, inflammation, toxicity, ethnobotany