Study on Effect of Aloe vera Leaf Extracts on Growth of Aspergillus flavus
Published: 2013-08-27
Page: 1091-1097
Issue: 2013 - Volume 3 [Issue 4]
A. Babaei *
Department of Biology, Faculty of Science, Malayer University, Malayer 65719-95863, Iran.
M. Manafi
Department of Animal Science, Faculty of Agricultural Science, Malayer University, Malayer 65719-95863, Iran.
H. Tavafi
Department of Biology, Faculty of Science, Malayer University, Malayer 65719-95863, Iran.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of this study is to evaluate and compare the antifungal activity of different extracts of Aloe vera plant on the growth of Aspergillus flavus.
Study Design: Considering the wide dispersal of Aspergillus flavus across the globe and its massive contamination on feed and food stuff of animal and human beings, it is inevitable to find a solution to inhibit the growth of this fungus and subsequently production of aflatoxin.
Place and Duration of Study: Department of Animal Science, Faculty of Agricultural Science, Malayer University, between August 2012 and March 2013.
Methodology: Six different solvents such as acetone, ethanol, water, methanol, chloroform and ethyl ether were employed for extraction from Aloe Vera fresh leaves. Antifungal activity of the extracts was evaluated by Agar Plate Diffusion Plate method.
Results: The maximum antifungal activity (100%) observed was in acetone extract group in concentration of 2000µL; the complete inhibition of fungus growth was found (100%).
Conclusion: The acetone extract of Aloe vera can be used as an effective antifungal agent to inhibit the growth of A. flavus compared to other solvents.
Keywords: Aloe vera, A. flavus, antifungal, aflatoxin